E-MTAB-1839 - Transcription profiling by array of mouse CGR8 ES cells with overexpression of Klf4 or Klf5 against GFP-overexpressing controls and wild-type controls to study the functional divergence of Klf4 and Klf5
Released on 28 April 2014, last updated on 13 May 2014
This experiment is a follow-up experiment of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, http://www.fungenes.org). The experiment was conducted at Inserm U846, Bron, France. Aim: Kru_ppel-like factors (Klf) 4 and 5 are two closely related members of the Klf family, known to play key roles in somatic cell reprogramming and in self-renewal of pluripotent stem cells. In this study, we focused on the functional divergence between Klf4 and Klf5. We showed that Klf4 and Klf5 regulate the expression of distinct subsets of genes. Klf4 negatively regulates the expression of endodermal markers, some of which encode transcription factors involved in the commitment of pluripotent system cells to endoderm differentiation. In contrast, Klf5 negatively regulates the expression of mesodermal markers, some of which controls commitment to the mesoderm lineage. Functional studies with reporter cell lines indicate that knockdown of Klf4 enhances differentiation toward visceral endoderm, mesendoderm, and definitive endoderm, whereas knockdown of Klf5 specifically enhances differentiation toward mesoderm. Thus, additive functions of Klf4 and Klf5 secure pluripotent stem cell propagation by inhibiting endoderm and mesoderm differentiation.
transcription profiling by array, strain or line, in vitro, co-expression
Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells. Irene Aksoy, Pierre-Yves Bourillot, Pierre Savatier et al.