E-MTAB-1833 - Transcription profiling by array of CUX1 knockdown in LOUCY cells lines
Released on 5 November 2013, last updated on 3 June 2014
LOUCY cells were resuspended in Opti-MEM (Gibco) and electroporated in 4 mm cuvettes (300 V, 1000 F; Genepulser XCell, Bio-Rad) using Stealth siRNAs (Ambion) against CUX1 or negative control duplexes. Cells were rested for 15 min following electroporation before dilution into normal RPMI 10 percent FBS growth media. RNA prepared from cells 48-72 h later using an RNeasy mini kit (Qiagen) was used for cDNA preparation for microarrays according to manufacturers instructions (Illumina).
transcription profiling by array, cellular modification design, co-expression design, in vitro design
Inactivating CUX1 mutations promotes tumorigenesis.