Title: Affymetrix CEL analysis. Description:
Transverse aortic constriction (TAC) or sham surgery was performed on 3 tamoxifen-injected Hand2F/F and 3 tamoxifen-injected MCM-Hand2F/F mice
nucleic acid labeling protocol
The assay started with 4Ð8 _g total RNA sample. The detection probes were made by in situ synthesis using photo-generated reagent chemistry. Hybridization used 100 _L 6 _ SSPE buffer (0.90 M NaCl, 60 mM Na2HPO4, 6 mM EDTA, pH 6.8) containing 25% formamide at 34¡C. After RNA hybridization, tag-conjugating Cy3 or Cy5 dyes were circulated through the microfluidic chip for dye staining.
nucleic acid extraction protocol
Cardiac RNA was extracted using Trizol
Cardiac RNA was collected from ventricular tissue of 3 tamoxifen-injected Hand2F/F and 3 tamoxifen-injected MCM-Hand2F/F mice (4 weeks after sham or TAC surgery)