E-MTAB-1794 - Amplicon sequencing-based profiling of formalin fixed paraffin embedded metastatic melanoma tissue samples
Released on 8 September 2013, last updated on 2 May 2014
Genetic heterogeneity can provide tumors with opportunities for therapy evasion, however the degree of genetic heterogeneity within metastatic melanomas has not been thoroughly investigated. We therefore isolated DNA from different regions of formalin fixed paraffin embedded metastatic melanoma tissue samples and subjected them to amplicon sequencing-based profiling of mutations in a panel of well known cancer genes using the Ion Ampliseq Cancer Panel.
genotyping by high throughput sequencing, disease state design, ex vivo, genotype design
Intratumoral genetic heterogeneity in metastatic melanoma is accompanied by variation in malignant behaviors. Anaka M, Hudson C, Lo PH, Do H, Caballero OL, Davis ID, Dobrovic A, Cebon J, Behren A. BMC Med Genomics 6:40 (2013), Europe PMC 24119551