E-MTAB-1789 - Transcription profiling by array of human p53-defective isogenic DLD1 diploid and tetraploid cell populations to study gene expression differences upon tetraploidisation

Status
Released on 22 April 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (5)
Description
Tetraploidisation has been implicated in tumorigenesis and in aneuploidy which is frequently observed in most human cancers. In this study we aim to reveal differences in gene expression profile of p53 defective isogenic diploid and tetraploid cell populations. Isogenic DLD1 populations were obtained by transient Dihydro-Cytochalasin B treatment and cell sorting of pure diploid and tetraploid populations by cell cycle profile several days after release from DCB. RNA isolation: Two technical replicas were collected for each population from two different passages (biological replicas) of 60-80% confluent cells.
Experiment types
transcription profiling by array, cell type comparison, co-expression, in vitro
Contact
Citation
APC/C is an essential regulator of centrosome clustering. Konstantinos Drosopoulos, Chan Tang, William C. H. Chao, Spiros Linardopoulos. Nat Commun 5
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1789.idf.txt
Sample and data relationshipE-MTAB-1789.sdrf.txt
Raw data (1)E-MTAB-1789.raw.1.zip
Array designA-GEOD-6790.adf.txt
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