E-MTAB-1766 - Nuclear depletion of the essential transcription termination factor Nrd1 in Saccharomyces cerevisiae was studied using a combination of RNA-Seq, ChIP-Seq of Pol II and PAR-CLIP of Nrd1

Status
Last updated on 13 November 2013, released on 14 November 2013
Organism
Saccharomyces cerevisiae
Samples (12)
Protocols (5)
Description
Nuclear depletion of the essential transcription termination factor Nrd1 in Saccharomyces cerevisiae was studied using a combination of RNA-Seq, ChIP-Seq of Pol II and PAR-CLIP of Nrd1. The drug rapamycin induces the formation of a ternary complex between a protein of interest, the drug and the small subunit of the ribosome (both proteins are genetically engineered). The small ribosome subunit is transported out of the nucleus. therefore the protein of interest can be depleted from nucleus upon treatment with rapamycin.
Experiment types
ChIP-seq, RNA-seq of coding RNA, PAR-CLIP, co-expression, compound treatment, in vivo, replicate design
Contact
Citation
Transcriptome Surveillance by Selective Termination of Noncoding RNA Synthesis. Schulz D, Schwalb B, Kiesel A, Baejen C, Torkler P, Gagneur J, Soeding J, Cramer P. Cell S0092-8674(13):01300-7 (2013)
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1766.idf.txt
Sample and data relationshipE-MTAB-1766.sdrf.txt
Links