7 protocols
AccessionNameType
 
P-MTAB-21678
nucleic_acid_extraction
RNA was extracted along with gDNA and protein using Qiagen AllPrep kits. (Parameters: Extracted product = total_RNA, Amplification = none)
P-MTAB-21676
grow
RF10 consists of basal RPMI 1640 (Invitrogen) with 2mM Glutamax (Invitrogen), 100U/ml Penicillin (Invitrogen), and 100ug/mL Streptomycin (Invitrogen), and 10% foetal calf serum (CSL, Melbourne, Australia). (Parameters: time unit = seconds, temperature un
P-MTAB-22219
labeling
Manufacturer’s (Illumina) standard protocol - 200ng starting material
P-MTAB-31026
image_acquisition
Beadchips were scanned using a Illumina Beadstation 500GX using default settings
P-MTAB-31025
hybridization
Manufacturer’s (Illumina) standard protocol - 48C for 17 hours
P-MTAB-31024
labeling
Manufacturer’s (Illumina) standard protocol - 200ng starting material