6 protocols
AccessionNameType
P-MEXP-9605
nucleic_acid_extraction
RNA was extracted using TriZol standard protocol
P-TABM-6231
bioassay_data_transformation
Microarray results are analyzed by GeneSpring GX 11 software (Agilent Technologies).Data transformation is applied to set a threshold at 1.0 of raw signals, followed by on-chip quantile normalization and subsequent transformation to base 2 logarithm.
P-TABM-6230
image_acquisition
Images at 5 um resolution are generated by Agilent scanner and the Feature Extraction 10.5 software is used to obtain the microarray raw-data.
P-TABM-6229
hybridization
Hybridizations are performed at 55 degrees C for 17 hours in a rotating oven.
P-TABM-6228
labeling
Labeled cRNA is synthesized from 500 ng of total RNA using the Low RNA Input Linear Amplification Kit (Agilent Technologies) in the presence of cyanine 3-CTP (Perkin-Elmer Life Sciences, Boston, MA).
P-MTAB-33572
treatment protocol
RNA was extracted from A375 cells, treated for 12 days with 20 uM of efavirenz or DMSO