6 protocols
array scanning protocol
Slides were scanned in double pass mode at 3 um resolution with a G2565CA microarray scanner (Agilent Technologies, Santa Clara, USA).
nucleic acid hybridization to array protocol
CytoChip Oligo reference manual v 1.8 (BlueGnome Ltd., Cambridge, UK)
nucleic acid labeling protocol
CytoChip Oligo reference manual v 1.8 (BlueGnome Ltd., Cambridge, UK)
treatment protocol
Specimens of dissected fetal tissue or cultured cells were thawed and washed three times in phosphate buffered saline (Invitrogen, Carlsbad, USA).
growth protocol
Chorionic villi or fetal tissue was carefully dissected and cultured for routine diagnostics. Samples of left over chorionic villi or cultured cells were stored in phosphate buffered saline (Invitrogen, Carlsbad, USA) at -20 degrees C.
nucleic acid extraction protocol
Genomic DNA was extracted utilizing the Maxwell(r)16 LEV Blood DNA kit (Promega Corp., Madison, USA), MagNA Pure kit (Roche, Grenzach-Wyhlen, Germany) or QIAamp DNA Blood Mini kit (QIAGEN, Hilden, Germany) according to respective manufacturers' protocols. Concentration and quality of genomic DNA was assessed spectrophotometrically (NanoDrop(r) ND 1000, peqlab Biotechnologie GmbH, Erlangen, Germany). If necessary, ethanol/sodium acetate precipitation was performed. High molecular weight of genomic DNA as well as complete restriction digestion were verified by agarose gel electrophoresis (AGE) according to standard laboratory protocols.