10 protocols
AccessionType
high throughput sequence alignment protocol
Aligned with Tophat2. We gave Tophat2 the grubii annotation and used the following parameters : --solexa1.3-quals --microexon-search --min-intron-length 30 --min-coverage-intron 30 --min-segment-intron 30 --max-intron-length 4000 --max-multihits 1
nucleic acid sequencing protocol
Sequencing on DNA libraries with the HiSeq 2000 Illumina device
nucleic acid extraction protocol
RNA was extracted with TRIZOL Reagent (Invitrogen) following the manufacturer's instructions
nucleic acid library construction protocol
TruSeq RNA sample prep protocol Illumina
growth protocol
at 30 degrees C in rich glucose containing medium (Yeast Extract Peptone Dextrose), cell density at 5 x10E7 cells/ml.
growth protocol
at 30 degrees C in rich glucose containing medium (Yeast Extract Peptone Dextrose) containing fuconazole (10 mg/l), cell density at 5 x10E7 cells/ml.
growth protocol
At 30 degrees C in rich medium (Yeast Extract Peptone Dextrose) containig SDS (0,01% w/v), cell density at 5 x10E7 cells/ml.
growth protocol
At 37 degrees C in rich glucose containing medium (Yeast Extract Peptone Dextrose), cell density at 5 x10E7 cells/ml.
growth protocol
at 30 degrees C in rich galactose containing medium (Yeast Extract Peptone Dextrose), cell density at 2 x10E8 cells/ml.
growth protocol
at 30 degrees C in rich glucose containing medium (Yeast Extract Peptone Dextrose), cell density at 2 x10E8 cells/ml.