E-MTAB-1695 - Transcription profiling by array of postnatal rat microglia and oligodendrocyte precusor cells with or without exposure to dexamethasone
Released on 8 March 2014, last updated on 3 June 2014
Primary mixed glial cultures were prepared from dissociated cerebral cortices of Sprague-Dawley rats at postnatal day 1 to 3 and glial populations were isolated by sequential rotary shaking procedures using well-established protocols. Cultured cells were exposed to 1 micromol dexamethasone for 24hours prior to RNA isolation. Control samples were exposed to equal volume of carrier. For each cell type, RNA samples from four cultures were dispatched to Source Bioscience UK Ltd. (Nottingham) for processing and hybridisation. RNA integrity was determined using the Bioanalyzer (Agilent) and the three RNA samples with the highest quality per condition were selected for microarray analysis. 750ng of processed cRNA was hybridised to Illumina RatRef-12 bead chips.
transcription profiling by array, all pairs, co-expression, compound treatment
Corticosteroid induced myelin delays are unlikely to be mediated via direct effects on oligodendrocyte lineage cells. Stuart I. Jenkins, Mark R. Pickard, Melinda Khong, Heather L. Smith, Carl L.A. Mann, Richard D. Emes, Divya M. Chari.