E-MTAB-1669 - CLIP-seq of Drosophila melanogaster clone 8 cells shows that two enzymes in the Male-Specific Lethal complex, MLE RNA helicase and MSL2 ubiquitin ligase, bind evolutionarily conserved domains containing tandem stem-loops in roX1 and roX2 RNAs in vivo

Status
Released on 18 July 2013, last updated on 3 May 2014
Organism
Drosophila melanogaster
Samples (6)
Protocols (8)
Description
Dosage compensation in Drosophila is an epigenetic phenomenon utilizing proteins and long non-coding RNAs (lncRNAs) for transcriptional up-regulation of the entire X-chromosome. Here, UV cross-linking followed by deep sequencing (iCLIP) show that two enzymes in the Male-Specific Lethal complex, MLE RNA helicase and MSL2 ubiquitin ligase, bind evolutionarily conserved domains containing tandem stem-loops in roX1 and roX2 RNAs in vivo.
Experiment types
CLIP-Seq, in vivo
Contacts
Citation
Tandem Stem-Loops in roX RNAs Act Together to Mediate X Chromosome Dosage Compensation in Drosophila. Ilik IA, Quinn JJ, Georgiev P, Tavares-Cadete F, Maticzka D, Toscano S, Wan Y, Spitale RC, Luscombe N, Backofen R, Chang HY, Akhtar A . Molecular Cell  (2013)
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1669.idf.txt
Sample and data relationshipE-MTAB-1669.sdrf.txt
Processed data (2)E-MTAB-1669.processed.1.zip, E-MTAB-1669.processed.2.zip
Links