E-MTAB-1657 - Comparative genomic hybridization by array to investigate ISBP mapping in the physical map of the Triticum aestivum chromosome arm 1BL

Status
Released on 1 June 2013, last updated on 3 June 2014
Organism
Triticum aestivum
Samples (1)
Array (1)
Protocols (6)
Description
To improve the resources for map-based cloning and sequencing of the wheat genome, we established a physical map of the wheat chromosome 1BL with a high density of markers by hybridizing the newly developed INRA GDEC Triticum aestivum NimbleGen 12x17k ISBP microarray (A-MEXP-2312) with BAC pools from the 1BL physical map. Then, we managed to map 3912 ISBP on the wheat chromosome 1BL BACs. The values in the 'Factor Value[individual]' column represent the BAC pool that have been hybridized on the array. For example, the assay 1 correspond to the hybridization of a bulk of all DNA BAC of the plate 1 of the MTP (Minimum Tilling path) BAC library of the chromosome 1BL.
Experiment types
comparative genomic hybridization by array, all pairs, genotyping design , normalization testing design
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Citation
A high density physical map of chromosome 1BL supports evolutionary studies, map-based cloning and sequencing in wheat. Philippe R., Paux E., Bertin I., Sourdille P., Choulet F., Laugier C., Simková H., Safá J., Bellec A., Vautrin S., Frenkel Z., Cattonaro F., Magni F., Scalabrin S., Martis M., Mayer K., Korol A., Bergès H., Doležel J., International Wheat Genome Sequencing Consortium, Feuillet C.
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