6 protocols
AccessionNameType
P-MTAB-32527
normalization data transformation protocol
read count normalization; factor calculated by (number of reads mapped to miRNA / 1 million)
P-MTAB-32526
high throughput sequence alignment protocol
novoalign v2.08.01; parameter: -a -l 15 -A ; reference genome hg19
P-MTAB-32525
nucleic acid sequencing protocol
TruSeq SBS v2; 36 bp single end
P-MTAB-32523
nucleic acid extraction protocol
Extraction of total RNA with TriReagent (Ambion) following manufacturers protocol
P-MTAB-32522
growth protocol
The samples were collected from the main surgical specimen at the time of surgery for colorectal cancer, and immediately snap frozen in liquid nitrogen.
P-MTAB-32524
nucleic acid library construction protocol
Following Illumina`s TruSeq smallRNA protocol; starting with 1 ug total RNA