E-MTAB-1628 - RNA-seq of coding RNA of mouse long term hematopoietic stem cells, short term hematopoietic stem cells and hematopoietic multipotent progenitor cells to investigate maternal-specific imprinting in the H19-Igf2 locus
Released on 12 August 2013, last updated on 3 May 2014
Long-term hematopoietic stem cells (HSCs), short-term HSCs and multipotent progenitor cells (MPPs) were isolated from bone marrow of four mouse strains (WT, H19-deletion, Igf1r-deletion, and double-deletion) and expression profiled with RNAseq. The behavior of the transcriptomes, and in particular the imprinted genes, was analyzed to see what might be involved in maintaining quiescence of long-term stem cells, and how H19 and Igf1r affected the expression of imprinted genes. Transcriptional profiling data of the same cells have been deposited in ArrayExpress under accession number E-MTAB-1644 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1644/).
RNA-seq of coding RNA, cell type comparison, co-expression, reference, strain or line
Maternal imprinting at the H19–Igf2 locus maintains adult haematopoietic stem cell quiescence. Venkatraman A, He XC, Thorvaldsen JL, Sugimura R, Perry JM, Tao F, Zhao M, Christenson MK, Sanchez R, Yu JY, Peng L, Haug JS, Paulson A, Li H, Zhong XB, Clemens TL, Bartolomei MS, Li L. Nature (2013), Europe PMC 23863936