6 protocols
AccessionNameType
P-ILMN-1
image_acquisition
image_acquisition
P-ILMN-2
hybridization
hybridization
P-MTAB-32289
nucleic acid labeling protocol
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
P-MTAB-32287
treatment protocol
Apart from ZHTc6 cells (which were treated with 1000ng/ml of Doxycyclin to maintain cells in the Oct4+/- state), all cell lines were grown under the same conditions with no special treatment.
P-MTAB-32288
nucleic acid extraction protocol
RNA was extracted with RNeasy Mini kit from Qiagen (Cat. No. 74104). Quality control was performed with Agilent Bioanalyser.
P-MTAB-32286
growth protocol
Mouse ES cells were grown in GMEMB/10% FCS with 100units/ml LIF on gelatinised tissue culture flasks.