6 protocols
AccessionNameType
P-MTAB-32761
normalization data transformation protocol
A variance stabilizing transformation and quantile normalized was performed using the lumi package in R
P-MTAB-32760
array scanning protocol
Illumina BeadScanner/BeadStation. Microarray Resources Centre, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
P-MTAB-32759
nucleic acid hybridization to array protocol
Standard protocol for hybridizing sample to Illumina Mouse WG-6 v2 expression BeadArrays. Performed at: Microarray Resources Centre, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
P-MTAB-32758
nucleic acid labeling protocol
Samples prepared and labelled according to standard Illumina protocol for hybridization to Mouse WG-6 v2 BeadArrays. Performed at: Microarray Resources Centre, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
P-MTAB-32757
nucleic acid extraction protocol
Total RNAs were purified using PureLink RNA Purification Kit (Invitrogen), 10-50ng RNA was pre-amplified (Nugen, Ovation RNA Amplification System)
P-MTAB-32756
sample treatment protocol
Cd34+ve, GFP positive and negative cells isolated from three mice. CD34+ve, Lrig1-eGFP positive and negative cells from back skin epidermis were isolated by flow cytomtry (Aria) from 8w Lrig1 KI animals