6 protocols
AccessionType
normalization data transformation protocol
A variance stabilizing transformation and quantile normalized was performed using the lumi package in R
array scanning protocol
Illumina BeadScanner/BeadStation. Microarray Resources Centre, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
nucleic acid hybridization to array protocol
Standard protocol for hybridizing sample to Illumina Mouse WG-6 v2 expression BeadArrays. Performed at: Microarray Resources Centre, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
nucleic acid labeling protocol
Samples prepared and labelled according to standard Illumina protocol for hybridization to Mouse WG-6 v2 BeadArrays. Performed at: Microarray Resources Centre, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
nucleic acid extraction protocol
Total RNAs were purified using PureLink RNA Purification Kit (Invitrogen), 10-50ng RNA was pre-amplified (Nugen, Ovation RNA Amplification System)
sample treatment protocol
Cd34+ve, GFP positive and negative cells isolated from three mice. CD34+ve, Lrig1-eGFP positive and negative cells from back skin epidermis were isolated by flow cytomtry (Aria) from 8w Lrig1 KI animals