E-MTAB-1605 - Transcription profiling by array of Danio rerio embryos with partially removed yolk to study the early-developmental responses to reduced nutrient availability
Released on 1 June 2013, last updated on 5 February 2016
Limited nutrient availability during development puts individuals at risk to develop complications later in life. Central in this early-life stress paradox lies developmental plasticity, a poorly understood mechanism that responds to environmental cues from early to late developmental stages. In this study, we aim to introduce the zebrafish (Danio rerio) as a model to study the early-developmental responses to reduced nutrient availability and their outcome. To reduce nutrient availability, we partially remove the yolk during embryogenesis. Around 5 hours post-fertilization, from 3 batches of embryos, we removed ~30% of the yolk (YD samples) or sham-punctured embryos (SP) with a Hamilton syringe system. At 2 days post-fertilization, we collected RNA from whole embryos and obtained transcriptome profiles by microarray hybridization.
transcription profiling by array, all pairs, co-expression, development or differentiation design, stimulus or stress