E-MTAB-1605 - Transcription profiling by array of Danio rerio embryos with partially removed yolk to study the early-developmental responses to reduced nutrient availability

Status
Released on 1 June 2013, last updated on 3 June 2014
Organism
Danio rerio
Samples (6)
Array (1)
Protocols (8)
Description
Limited nutrient availability during development puts individuals at risk to develop complications later in life. Central in this early-life stress paradox lies developmental plasticity, a poorly understood mechanism that responds to environmental cues from early to late developmental stages. In this study, we aim to introduce the zebrafish (Danio rerio) as a model to study the early-developmental responses to reduced nutrient availability and their outcome. To reduce nutrient availability, we partially remove the yolk during embryogenesis. Around 5 hours post-fertilization, from 3 batches of embryos, we removed ~30% of the yolk (YD samples) or sham-punctured embryos (SP) with a Hamilton syringe system. At 2 days post-fertilization, we collected RNA from whole embryos and obtained transcriptome profiles by microarray hybridization.
Experiment types
transcription profiling by array, all pairs, co-expression, development or differentiation design, stimulus or stress
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1605.idf.txt
Sample and data relationshipE-MTAB-1605.sdrf.txt
Raw data (1)E-MTAB-1605.raw.1.zip
Processed data (2)E-MTAB-1605.processed.1.zip, E-MTAB-1605.processed.2.zip
Array designA-GEOD-14664.adf.txt
Links