E-MTAB-1595 - ChIP-seq of budding yeast cells for the genomic maps of Mediator, Rad2, Rad3, RNA Pol II, TBP and TFIIH to study the functional link between Rad2 and Mediator
Released on 1 December 2013, last updated on 3 May 2014
In this study, we generate genomic maps of Mediator, Rad2, Pol II, TBP and TFIIH, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae. A related study involving ChIP-chip analysis of Rad2 occupany is also deposited at ArrayExpress under accession number E-MEXP-3875 ( http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-3875 ).
ChIP-seq, ChiP-seq, binding site identification, in vivo, individual genetic characteristics, strain or line
Mediator links transcription and DNA repair by facilitating Rad2/XPG recruitment. Fanny Eyboulet, Camille Cibot, Thomas Eychenne, Helen Neil, Olivier Alibert, Michel Werner, and Julie Soutourina. Genes Dev. 27:2549-2562 (2013)