5 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Title: Affymetrix CEL analysis. Description:
P-AFFY-2
labeling
RNA was isolated using the RNeasy Plant Mini Protocol (QIAGEN, Clifton Hill, Australia) and the quality of the RNA was assessed using the Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA) where spectrophotometric analysis was carried out to determine the A260 to A280 ratio and the concentration. For qRT-PCR, cDNA synthesis was carried out using the Biorad (Perth) iScript cDNA synthesis kit according to manufacturers instructions. For arrays, the cRNA was prepared from 3 ug of total RNA almost exactly as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
P-AFFY-3
hybridization
Title: GeneChip hybridization. Description:
P-MTAB-32171
nucleic acid extraction protocol
Standard Affymetrix protocol for tota RNA isolation
P-MTAB-32170
growth protocol
Cells from spleen were prepared for flow cytometry as previously described (Chatzidakis, I et al., 2007. An essential role for tnf in modulating thresholds for survival, activation, and tolerance of cd8+ t cells. J Immunol 178:6735-45)