E-MTAB-1557 - RNA-seq of non coding RNA of mouse Mili_Ctl and Mili_C-DAH testes to investigate epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis

Status
Last updated on 3 July 2013, released on 3 July 2013
Organism
Mus musculus
Samples (4)
Protocols (5)
Description
The Piwi protein Mili and the Piwi-interacting RNA (piRNA) pathway are required to establish L1 DNA methylation during epigenetic reprogramming that accompanies male germ cell development. Although Mili is expressed throughout most of adult spermatogenesis, its function therein remains unknown. We find that Mili displays a hitherto unappreciated dynamic expression profile during spermatogenesis, expressed in the mitotic spermatogonia but absent at the early meiotic stages only to reappear in late zygotene spermatocytes. Here we show using conditional mutagenesis that Mili's endonuclease activity post-transcriptionally cleaves L1 transcripts commencing upon its reappearance in late zygotene spermatocytes even in the presence of normal L1 DNA methylation
Experiment types
RNA-seq of non coding RNA, genetic modification, in vivo, individual genetic characteristics, microRNA profiling by high throughput sequencing, replicate
Contact
Citation
Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis. Monica Di Giacomo, Stefano Comazzetto, Harpreet Saini, Serena De Fazio, Claudia Carrieri, Marcos Morgan, Lina Vasiliauskaite, Vladimir Benes, Anton J. Enright, Donal O'Carroll. 50(4):601-608 (2013), Europe PMC 23706823
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1557.idf.txt
Sample and data relationshipE-MTAB-1557.sdrf.txt
Links