E-MTAB-1552 - ChIP-chip by array of TAP-tagged Saccharomyces cerevisiae strains to elucidate the general initiation-elongation transition of Pol II
Last updated on 29 July 2013, released on 30 July 2013
After transcription initiation, RNA polymerase (Pol) II escapes from the promoter and recruits elongation factors. Here we used chromatin immunoprecipitation (ChIP) to elucidate the general initiation-elongation transition of Pol II in yeast. We extended our ChIP-chip occupancy profiling to capping enzymes and the cap-binding complex (CBC). We found that the first two capping enzymes, Cet1 and Ceg1, are recruited near the TSS, whereas the third enzyme, the methyltransferase Abd1, is recruited about 110 nt downstream of the TSS, and CBC is recruited further downstream. ChIP was performed using TAP-tagged S. cerevisiae strains (see protocols).
ChIP-chip by array, binding site identification design, cellular process, in vivo
Cap completion and CTD kinase recruitment govern the initiation-elongation transition of RNA polymerase II.