7 protocols
AccessionNameType
P-MTAB-31931
normalization data transformation protocol
miRNA signal intensities from GeneView files were subjected to quantile normalization
P-MTAB-31930
array scanning protocol
Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner at a resolution of 2 micron.
P-MTAB-31929
nucleic acid hybridization to array protocol
Slides were hybridized for 20 h at 55°C using an Agilent hybridization system.
P-MTAB-31928
nucleic acid labeling protocol
Total RNA samples (100 ng) containing miRNA were labeled with cyanine 3-pGp (Cy3) using the Agilent microRNA Complete Labeling and Hyb Kit.
P-MTAB-31926
treatment protocol
Old mice were subdivided into two groups and fed different diets based on a modified AIN 76 diet: H-EVOO group was fed a diet in which the lipid component was provided by an extra-virgin olive oil high in phenolic antioxidants (718.8 mg/kg of total polyphenols); L-EVOO group by the same extra-virgin olive oil deprived of phenolic compounds (9.3 mg/kg of total polyphenols). Mice were sacrificed after 6 or 12 months of treatment. Male C57Bl/6J and TgCRND8 mice were sacrificed at 3-6 months of age. Brains were removed, dissected and immediately frozen in liquid nitrogen and stored at - 80 C.
P-MTAB-31925
growth protocol
Male C57Bl/6J mice aged 10 months at the beginning of the experiment were fed for 6 or 12 months with the experimental diets; young C57Bl/6J mice (4-6 months) and transgenic hemizygous TgCRND8 mice (3 months of age) were fed with a Standard lab chow.
P-MTAB-31927
nucleic acid extraction protocol
Total RNA was isolated from mice brain area by using the Absolutely RNA miRNA Kit (Agilent) according to the manufacturer’s protocol.