5 protocols
AccessionNameType
P-MTAB-31571
image_acquisition
Arrays were scanned using an Agilent C scanner at 5um resolution (20 bit scan).
P-MTAB-31570
hybridization
Labeled RNA samples were normalized at 700ng of each dye component, fragmented and hybridized to a custom anti-sense probe 2x11K microarray (AMADID 015123) using Agilent buffers.
P-MTAB-31569
labeling
Pooled total RNA samples were directly labeled (no amplification) with Kreatech ULS aRNA Labeling Kit.
P-MTAB-31567
grow
WT and delta P3 cells from plates were grown in liquid BG-11 for 4 days in PA LL conditions. They were re-suspended to about 1x107 cells ml-1 in 100 ml of BG-11 with 25 mM Hepes-NaOH pH 7.5, then grown for 1.25 days in photoautotrophic conditions till the cell count reached around the mid-log level. 5 mM glucose was then added and the cultures were grown for another 2 days in 12LD cycles. Samples for RNA extraction were taken after adding glucose at 1 h in day 1 in the light (L1) and in the dark (D1), and 1h in day 2 in the light (L13), and in the dark (D13). Two biological replicates and four technical replicates were used per time point per culture including a dye swap.
P-MTAB-31568
nucleic_acid_extraction
Cells from the four time points were spun down and the pellet was frozen in STET buffer at -80?C. RNA was extracted using Tri-reagent (Ambion), Dnase-treated, and column purified (Zymo research).