5 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Title: Affymetrix CEL analysis. Description:
P-AFFY-3
nucleic acid hybridization to array protocol
GeneChip hybridization
P-MTAB-28867
nucleic acid labeling protocl
WT-Ovation RNA Amplification System (NuGEN Technologies Inc., San Carlos, CA, USA). The reaction products were purified with a QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany).
(Parameters: Amplification = PCR, Mass unit = Micro gram)
P-MTAB-28865
nucleic acid extraction protocol
Frozen PAXgeneTM Blood RNA tubes were thawed at room temperature for 3 hours, followed by total RNA extraction with the PAXgeneTM Blood RNA kit according to the manufacturer's instruction.
(Parameters: Extracted product = total_RNA, Amplification = none)
P-MTAB-31401
growth protocol
For each collection, 2.5 ml of peripheral blood was drawn into a PAXgene Blood RNA tube (PreAnalytiX GmbH, Hombrechtikon, CH) and stored at -80 degrees C.