7 protocols
AccessionNameType
P-MTAB-31561
normalization data transformation protocol
Feature Extraction version 10.7 was used to filter, normalize, and calculate the signal intensities and Cy5/Cy3 ratios.
P-MTAB-31560
array scanning protocol
Axon Genepix 4000B microarray scanner at 5 micrometer resolution. Data from both channels were extracted using the Agilent Feature Extractor Software, Version 10.7
P-MTAB-31559
nucleic acid hybridization to array protocol
18 hour incubation at 65°C and 10 rpm in an Agilent Hybridization oven (Agilent p/n G2545A)
P-MTAB-31558
nucleic acid labeling protocol
We used the Agilent Two-Color Microarray-Based Gene Expression Analysis version 5.7 protocol
P-MTAB-31557
nucleic acid extraction protocol
Total RNA was isolated from mice brain area by using the Absolutely RNA miRNA Kit (Agilent) according to the manufacturer’s protocol.
P-MTAB-31556
treatment protocol
The animals were randomly subdivided into two groups and fed different diets (about 3 grams/mouse per day).We used a modified AIN 76 diet, with lipids providing about 21% of the total energy, composed of (in grams/100 grams of diet): 44.3 sucrose, 10.0 olive oil, 21.0 casein, 14.0 corn starch, 5.4 cellulose, 3.7 AIN 76 Mineral mix, 1.1 AIN 76 Vitamin mix, 0.3 methionine, and 0.2 choline. H-EVOO group was fed a diet in which the lipid component was provided by an extra-virgin olive oil high in phenolic antioxidants (718.8 mg/kg of total polyphenols); L-EVOO group by the same extra-virgin olive oil deprived of phenolic compounds (9.3 mg/kg of total polyphenols).Mice were sacrificed after 6 months of treatment. Brains were removed, dissected and immediately frozen in liquid nitrogen and stored at - 80 C.
P-MTAB-31555
growth protocol
Male C57Bl/6J mice aged 10 months at the beginning of the experiment were fed for 6 months with the experimental diets. Young C57Bl/6J mice (4-6 months) were also used as reference.