6 protocols
AccessionNameType
P-MTAB-31114
scanning
Sequencing was performed using an Illumina Genome Analyzer II platform. Single reads of 51bp were generated.
P-MTAB-31113
sequencing
Anti GATA-1 ChIPed DNA from Ter119-, Ter119+ and a 'no antibody' (input DNA) were processed for deep sequencing using the Illumina Genome Analyzer II platform according to Illumina protocols (www.illumina.com). Deep sequencing was carried out in duplicate for each ChIP sample and once for Input DNA controls producing 51-nuleotide sequence reads.
P-MTAB-31115
nucleic acid library construction protocol
ChIPed and INPUT DNA libraries were constructed according to Illumina protocols (http://www.illumina.com).
P-MTAB-31112
nucleic_acid_extraction
Formaldehyde-crosslinked chromatin from 10^7 Ter119- or Ter119+ fetal liver cells was prepared as previously described (Schuh et al, 2005)
P-MTAB-31110
grow
Fetal liver cells from day E12.5 C57/BL6 mouse embryos expanded for 3 days in serum free medium (von Lindern et al, 2001)
P-MTAB-31111
specified_biomaterial_action
Fractionation of Ter119- and Ter119+ cells with Miltenyi beads (Schuh et al, 2005)