E-MTAB-1495 - miRNA-Seq of human MCF-7 cells expressing or not ER-beta in absence of estrogen stimuli

Status
Released on 9 April 2014, last updated on 3 May 2014
Organism
Homo sapiens
Samples (2)
Protocols (6)
Description
We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from MCF-7 cells expressing or not human Erbeta in absence of estrogen stimuli was subjected to size selection labrary preparation ond retrotranscribed. Obtained DNA was sequenced with the Illumina/Solexa Genome Analyzer (GAIIX).
Experiment types
RNA-seq of non coding RNA, high throughput sequencing
Contacts
Citation
Post-transcriptional regulation of human breast cancer cell proteome by unliganded Estrogen Receptor beta via microRNAs. Giovanni Nassa, Roberta Tarallo, Giorgio Giurato, Maria Rosaria De Filippo, Maria Ravo, Francesca Rizzo, Claudia Stellato, Marc Baumann, Niina Lietzèn, Tuula A. Nyman and Alessandro Weisz.
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1495.idf.txt
Sample and data relationshipE-MTAB-1495.sdrf.txt
Links