6 protocols
AccessionNameType
P-MTAB-33051
bioassay_data_transformation
Raw data files were quantile normalized accross all chips. Normalised data are provided as an additional file (all.qnorm_me.txt) in this submission.
P-MTAB-33050
array scanning protocolnormalization data transformation protocol
AgilentG3 miRNA scan protocol with feature extraction software 10
P-MTAB-33049
nucleic acid hybridization to array protocol
Agilent miRNA microarray system with miRNA complete labelling and Hybridization standard Protocol
P-MTAB-33048
nucleic acid labeling protocol
Agilent miRNA microarray system with miRNA complete labelling and Hybridization standard Protocol
P-MTAB-33046
growth protocol
During routine screening by teams of the WHO control program in West Africa, indigens of sleeping sickness foci were screened with the Card Agglutination Test for Trypanosomiasis (CATT). CATT positive individuals were futher screened with CATT on plasma and confirmed by parasitological examination after parasite concentration. Stage determination was achieved via white cell count.
P-MTAB-33047
nucleic acid extraction protocol
Total RNA was extracted from all samples using TriFAST peqGolD reagent as described by the manufacturer.