4 protocols
Illumina Genome Analyzer IIx
nucleic acid sequencing protocol
ILLUMINA sequencing using Illumina Genome Analyzer IIx. http://www.illumina.com/support/sequencing/sequencing_instruments.ilmn
nucleic acid library construction protocol
Libraries were prepared from 10ng of ChIP-enriched DNA using Illumina's ChIP-seq DNA sample preparation kit. This was sequenced to generate single end 40bp reads.
growth protocol
Neural stem cells cultured in either proliferation-promoting medium (proNSC) or quiescence-promoting medium (quiNSC)
nucleic acid extraction protocol
Chromatin Immunoprecipitation with antibodies to H3K27ac (Abcam, ab4729), p300 (Santa Cruz, sc-585) and NFI transcription factors (Santa Cruz, sc-30918).