5 protocols
AccessionNameType
P-TABM-3122
nucleic_acid_extraction
To extract total RNA yeast cells were lysed by vortexing with glass beads in the presence of hot acid phenol. RNA was then cleaned by phenol:chlorophorm extraction followed by ethanol precipitation. polyA RNA isolation with subsequent cDNA synthesis with dUTP incorporation and in presence of actinomycin D.
P-TABM-3126
labeling
Fragmentation and biotinylation with Affymetrix GeneChip WT Terminal labeling kit.
P-TABM-4416
image_acquisition
Scanner type: GeneChip 3000 7G. Scanning software: default software
P-TABM-4415
hybridization
Modified tiling array Affy target Hybridization with GeneChip control oligoB2, Affymetrix.
P-MTAB-30039
grow
For YPD_BPS and YPMalt:Grow single colony in 3 ml YPD for the day at 30C. Dilute in 100 ml of desired medium with starting OD600=0.0019. Grow to OD600=1.0 at 30C. Harvest cells for total RNA extraction.for YPD_Rapa/YPE:Grow single colony overnight in 5 ml YPD at 30C. Dilute in 30 ml of desired medium with starting OD600 = 0.25. Grow the culture to OD600=0.9 for the day. Incubate the day culture in 400 ml of desired medium at 30C overnight. Harvest cells at OD600 at 1.0 for total RNA extraction.