10 protocols
AccessionType
normalization data transformation protocol
Robust Multi Array (RMA) data processing and summarization protocol using R/Bioconductor packages. Each tissue (parenchyma and nasal epitheilium) was normalized individually and summarized in one expression matrix.
array scanning protocol
Affymetrix scanning according to suppliers GeneTitan protocol
feature extraction
Affymetrix GeneTitan feature extraction according to suppliers protocol
nucleic acid hybridization to array protocol
Affymetrix hybridization according to suppliers GeneTitan protocol
nucleic acid labeling protocol
Affymetrix GeneTitan labeling protocol by biotinylation
nucleic acid extraction protocol
Transcriptome analysis was done following the manufacturer’s recommendations in the GeneChip® HT 3' IVT Express Kit (Santa Clara, CA) guide.
specified_biomaterial_action
Minikit cell lysis protocol for mRNA and miRNA processing workflows
dissect
Organ extraction and whole tissue dissection by 3x3 slices
compound based treatment
Animals were exposed to fresh air for 1, 2, and 3 months
compound based treatment
Animals were exposed to either cigarette smoke or fresh air for 3, 4, 5, and 6 months