4 protocols
AccessionNameType
P-MTAB-30000
scanning
The 100bp paired-end reads obtained by RNA-Seq were compiled using manufacturer-provided pipeline software CASAVA 1.8.2. Reads were then aligned onto the equine chromosomes with TOPHAT 1.3.2 using default settings.
P-MTAB-29998
nucleic_acid_extraction
For RNA extraction the Guanidium-thiocyanate-phenol-chloroform technique, with ethanol extraction was used (Chomczynski, P. and N. Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987
P-MTAB-29999
sequencing
Illumina HiSeq 2000
P-MTAB-29997
specified_biomaterial_action
Full thickness cartilage was removed from the entire surface of MCPII.All samples were scored macroscopically to determine any pathological abnormalities of the distal condyles of the distal third metacarpus.