E-MTAB-1381 - Transcription profiling by array of siderophore-drug resistant mutant of Pseudomonas aeruginosa PAO1 in order to obtain information on resistance mechanism(s)

Status
Released on 17 April 2013, last updated on 3 May 2014
Organism
Pseudomonas aeruginosa
Samples (9)
Array (1)
Protocols (8)
Description
The aim of the experiment was to identify genes differentially expressed between the susceptible wild type strain P. aeruginosa PAO1 (PT5) and a mutant resistant to a drug-siderophore conjugate, in order to obtain information on the resistance mechanism(s). A mutant of PT5 able to grow at 4 mg/l BAL30072, a drug-siderophore conjugate, was selected in vitro . The susceptible wild type strain PT5 and the mutant (BAL6) were grown in LB medium and the mutant also in the presence of 4 mg/l BAL30072 to mid-exponential growth phase (OD600 =2) in triplicate cultures. RNA was extracted using the RNeasy Kit (Qiagen). A total of nine Affymterix P. aeruginosa arrays were hybridized (one for each replicate) under standard conditions.
Experiment types
transcription profiling by array, co-expression, compound treatment, reference
Contact
Citation
Involvement of Fe Uptake Systems and AmpC beta-Lactamase in Susceptibility to the Siderophore Monosulfactam BAL30072 in Pseudomonas aeruginosa. Christian van Delden; Malcolm G. P. Page; Thilo Köhler. Antimicrobial Agents Chemother (Bethesda) 57(5):2095 (2013), Europe PMC 23422914
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1381.idf.txt
Sample and data relationshipE-MTAB-1381.sdrf.txt
Raw data (1)E-MTAB-1381.raw.1.zip
Processed data (1)E-MTAB-1381.processed.1.zip
Array designA-AFFY-30.adf.txt
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