E-MTAB-1381 - Transcription profiling by array of siderophore-drug resistant mutant of Pseudomonas aeruginosa PAO1 in order to obtain information on resistance mechanism(s)
Released on 17 April 2013, last updated on 3 May 2014
The aim of the experiment was to identify genes differentially expressed between the susceptible wild type strain P. aeruginosa PAO1 (PT5) and a mutant resistant to a drug-siderophore conjugate, in order to obtain information on the resistance mechanism(s). A mutant of PT5 able to grow at 4 mg/l BAL30072, a drug-siderophore conjugate, was selected in vitro . The susceptible wild type strain PT5 and the mutant (BAL6) were grown in LB medium and the mutant also in the presence of 4 mg/l BAL30072 to mid-exponential growth phase (OD600 =2) in triplicate cultures. RNA was extracted using the RNeasy Kit (Qiagen). A total of nine Affymterix P. aeruginosa arrays were hybridized (one for each replicate) under standard conditions.
transcription profiling by array, co-expression, compound treatment, reference
Involvement of Fe Uptake Systems and AmpC beta-Lactamase in Susceptibility to the Siderophore Monosulfactam BAL30072 in Pseudomonas aeruginosa. Christian van Delden; Malcolm G. P. Page; Thilo Köhler. Antimicrobial Agents Chemother (Bethesda) 57(5):2095 (2013), Europe PMC 23422914