E-MTAB-1361 - Transcription profiling by array of human pre-treatment samples of pathologically confirmed solitary fibrous tumor
Released on 12 November 2013, last updated on 3 May 2014
Sixteen pre-treatment samples of pathologically confirmed solitary fibrous tumors (SFT) were available for RNA profiling. They were collected from 16 patients who underwent initial surgery and/or diagnostic biopsy. Samples were macrodissected by pathologists, and frozen within 30 min of removal in liquid nitrogen in our biobank (Biobank authorization number 2008/70, APHM). All profiled specimens contained more than 70% tumor cells. Each patient gave written informed consent for molecular analysis, and the study was approved by our institutional ethics committee. Total RNA was extracted from frozen samples by using the All-In-One Norgen Biotek kit (Thorold, Canada) and integrity was controlled by Agilent analysis (Bioanalyzer, Palo Alto, CA). Gene expression profiling was done with Affymetrix U133 Plus 2.0 human oligonucleotide microarrays with labeling kit and protocol from manufacturer.
transcription profiling by array, clinical history, co-expression, disease state design
Gene expression profiling of solitary fibrous tumors shows potential stem cell features. François BERTUCCI, Corinne BOUVIER-LABIT, Pascal FINETTI, Philippe METELLUS, Karima MOKHTARI, Anne-Valérie DECOUVELAERE, Catherine MIQUEL, Dominique FIGARELLA-BRANGER, Jean-Michel COINDRE, Daniel BIRNBAUM.