E-MTAB-1349 - Transcription profiling by array of human T-ALL cells purified from the BM of mice treated with anti-Notch1 or control mAbs
Last updated on 15 November 2013, released on 15 November 2013
We generated PDTALL19 xenograft by intravenously injection of primary T-ALL cells from BM of a newly diagnosed ALL pediatric patient in NOD/SCID mice. To test the effect of Notch1 blockade on PDTALL19 cells engraftment, mice were treated with anti-human Notch1 mAb OMP-52M51 (OncoMed Pharmaceuticals Inc., Redwood, CA) or control antibody (Rituximab, Roche, Basel, Switzerland) (6 mice/group). Three independent experiments (1, 2, 3) were performed. In each experiment T-ALL cells from the BM of different mice were pooled, sorted and finally RNA was extracted. Differential expression analysis identified 194 up- and 209 down-regulated genes (BH-FDR<0.05 and absolute FC>1.5) in anti-Notch1 vs. control Ab treated samples.
transcription profiling by array, co-expression, compound treatment, in vivo
Therapeutic antibody targeting of Notch1 in T-acute lymphoblastic leukemia xenografts. Agnusdei V, Minuzzo S, Frasson C, Grassi A, Axelrod F, Satyal S, Gurney A, Hoey T, Seganfreddo E, Basso G, Valtorta S, Moresco R, Amadori A, Indraccolo S. Leukemia (2013), Europe PMC 23774673