E-MTAB-1343 - Transcription profiling by array of human embryonic stem cells differentiated to early Pax6 positive neural epithelial progenitor cells and differentiated further in neurospheres
Released on 8 November 2013, last updated on 3 May 2014
Human embryonic stem cells were differentiated to early Pax6 positive neural epithelial progenitor cells and were differentiated further in neurospheres. On indicated days (hESC: n=3, day 0: n=3, day 5 n=3, day 15: n=2, day 22: n=1 and day 25: n=1) cells were extracted, mRNA prepared and hybridized in triplicates to Illumina Sentrix HumanHT-12 BeadChip gene arrays. More experimental detail is available under Hoelting et al., Archives of Toxicology.
transcription profiling by array, development or differentiation, replicate, time series
A 3-dimensional human embryonic stem cell (hESC)-derived model to detect developmental neurotoxicity of nanoparticles (Nano-DNT). Lisa Hoelting, Benjamin Scheinhardt, Olesja Bondarenko, Stefan Schildknecht, Marion Kapitza, Vivek Tanavde, Betty Tan, Stefan Mecking, Marcel Leist, Suzanne Kadereit.