E-MTAB-1343 - Transcription profiling by array of human embryonic stem cells differentiated to early Pax6 positive neural epithelial progenitor cells and differentiated further in neurospheres

Status
Released on 8 November 2013, last updated on 3 May 2014
Organism
Homo sapiens
Samples (13)
Array (1)
Protocols (6)
Description
Human embryonic stem cells were differentiated to early Pax6 positive neural epithelial progenitor cells and were differentiated further in neurospheres. On indicated days (hESC: n=3, day 0: n=3, day 5 n=3, day 15: n=2, day 22: n=1 and day 25: n=1) cells were extracted, mRNA prepared and hybridized in triplicates to Illumina Sentrix HumanHT-12 BeadChip gene arrays. More experimental detail is available under Hoelting et al., Archives of Toxicology.
Experiment types
transcription profiling by array, development or differentiation, replicate, time series
Contact
Citation
A 3-dimensional human embryonic stem cell (hESC)-derived model to detect developmental neurotoxicity of nanoparticles (Nano-DNT). Lisa Hoelting, Benjamin Scheinhardt, Olesja Bondarenko, Stefan Schildknecht, Marion Kapitza, Vivek Tanavde, Betty Tan, Stefan Mecking, Marcel Leist, Suzanne Kadereit.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1343.idf.txt
Sample and data relationshipE-MTAB-1343.sdrf.txt
Raw data (1)E-MTAB-1343.raw.1.zip
Array designA-MEXP-2072.adf.txt
Links