7 protocols
AccessionNameType
P-MTAB-29411
data acquisition
The intensity files were loaded into GenomeStudio v.2010.3 software for quality control and gene expression analysis
P-MTAB-29410
scanning
BeadChips were dried and subsequently scanned with an Illumina BeadArray Reader
P-MTAB-29409
hybridization
Hybridization reactions were performed for 18 hours on Illumina HumanHT12 v.4.0 Expression BeadChips
P-MTAB-29408
labeling
Total RNA were fluorescently labelled, amplified and hybridized in triplicate
P-MTAB-29406
treatment
K-562 cells were used to produce five distinct cell cultures: wild type, FHC-silenced, FHC-reconstituted,wild type hemin-treated, FHC-silenced hemin-treated
P-MTAB-29407
extraction
Cells were lysed and RNA was extracted using TRIzol method, according to manufacturer's instruction
P-MTAB-29405
grow
K-562 cells were cultured in standard growth condition