E-MTAB-1295 - Transcription profiling by array of gene expression changes during infection in Phytophthora capsici and tomato (Solanum lycopersicum) to investigate changes in both host and pathogen transcriptomes
Last updated on 4 December 2012, released on 24 March 2013
Phytophthora capsici, Solanum lycopersicum
Phytophthora species are a destructive group of filamentous plant pathogens, which have a global distribution and devastating effect on a wide range of plants important to agriculture and natural ecosystems. Throughout the infection cycle, Phytophthora secrete an effector repertoire into its host to inhibit or counter defence associated compounds, lytic enzymes and intracellular processes required for immunity. Despite the advent of genome sequences of both host and microbe however, little is known about the signal interplay between host and pathogen during infection. Here we explore and report on the association between the hemi-biotrophic broad host range pathogen Phytophthora capsici and tomato. Infection assays reveal a distinct hemi-biotrophic infection cycle, featuring haustoria formation early in infection, followed by necrotrophy in the lateinfection stages. We assessed gene expression changes during infection in both P. capsici and tomato and unveil distinct changes in both host and pathogen transcriptomes, associated with biotrophy and the subsequent switch to necrotrophy. These results suggest dynamic but highly regulated transcriptional programmes that underpin P. capsici hemi-biotrophy. Our results provide new detail on coordinate transcriptional reprogramming during infection and sheds light on the basic processes that accompany hemibiotrophy. Please note the timepoint is hours post inoculation (hpi) of P capsici zoospores on detached tomato leaves. Where it is na (not applicable), these are in vitro cultures of P capsici only.
transcription profiling by array, co-expression, disease state, pathogenicity, time series
Phytophthora capsici-tomato host interaction features dramatic and coordinated shifts in host and pathogen gene expression. Julietta Jupe, Jenny Morris, Remco Stam, Andrew Howden, Pete Hedley and Edgar Huitema.