E-MTAB-1264 - Transcription profiling by array of Arabidopsis whole plants and discrete root, hypocotyl and shoot responses to the orbital environment
Submitted on 4 October 2012, released on 4 September 2013, last updated on 27 March 2015
Arabidopsis thaliana was evaluated for its response to the spaceflight environment in three replicated experiments on the International Space Station. Two approaches were used; GFP reporter genes were used to collect gene expression data in real time within unique GFP imaging hardware, and plants were harvested on orbit to RNAlater for subsequent analyses of gene expression with using Affymetrix and SAGE transcriptome analyses. Three tissue types were examined (leaves, hypocotyls and roots) and compared to analyses conducted with whole plants. Transcriptome analyses with whole plants suggested that the spaceflight environment had little impact on the transcriptome of arabidopsis, however, closer examination of selected tissues revealed that there are a number of tissue-specific responses that arabidopsis employs to respond to this novel environment. This experiment was launched on STS-131 in 2010 and was supported by NASA grant NNX07AH27G - Transgenic Plant Biomonitors of Space Flight Exposure to R.J. Ferl and A-L. Paul.
transcription profiling by array, genetic modification, individual genetic characteristics
Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight. Paul AL, Zupanska AK, Schultz ER, Ferl RJ. BMC plant biology :112 (2013), Europe PMC 23919896