E-MTAB-1234 - Transcription profiling by array of human AGS cell line cultured in standard 2D conditions or on 3D matrigel membranes
Released on 15 February 2013, last updated on 3 May 2014
AGS cell line was cultured in standard T25 flasks (2D condition) and in T25 flasks precoated with 50 µL/cm2 of matrigel basement membrane (BD Biosciences) at a 1.5:1 proportion to RPMI serum-free medium (3D condition, overlay method). Cultures were maintained for 14 days with medium change every two days. Transcriptome alterations were then investigated.
transcription profiling by array, growth condition design
CDX2 regulation by the RNA-binding protein MEX3A: impact on intestinal differentiation and stemness. Bruno Pereira; Sofia Sousa; Rita Barros; Laura Carreto; Patrícia Oliveira; Carla Oliveira; Nicolas T. Chartier; Michelina Plateroti; Jean-Pierre Rouault; Jean-Noël Freund; Marc Billaud; Raquel Almeida. Nucleic Acids Res 41(7):3986 (2013), Europe PMC 23408853