5 protocols
AccessionNameType
P-MTAB-27174
image_acquisition
Scanning was carried out by the SBMRI microarray facility.
P-MTAB-27173
hybridization
Hybridization was carried out by the SBMRI microarray facility.
P-MTAB-27172
labeling
Labelling was carried out by the SBMRI microarray facility.
P-MTAB-27170
grow
Standard mES culture (15%FBS, 1%NEAA and Glutamine, with LIF and thioglycerol, 37'C 5%CO2), for iPSCs, they were infected with 4F for 3 days and cultured in mES medium until day 14, medium was refreshed every other day
P-MTAB-27171
nucleic_acid_extraction
TRIZOL protocol, standard(1ml Trizol per well for 6-well plate, all the other steps are the same as suggested by manufacteruer (catalog number: 15596-026, Invitrogen)