5 protocols
AccessionType
image_acquisition
Scanning was carried out by the SBMRI microarray facility.
hybridization
Hybridization was carried out by the SBMRI microarray facility.
labeling
Labelling was carried out by the SBMRI microarray facility.
grow
Standard mES culture (15%FBS, 1%NEAA and Glutamine, with LIF and thioglycerol, 37'C 5%CO2), for iPSCs, they were infected with 4F for 3 days and cultured in mES medium until day 14, medium was refreshed every other day
nucleic_acid_extraction
TRIZOL protocol, standard(1ml Trizol per well for 6-well plate, all the other steps are the same as suggested by manufacteruer (catalog number: 15596-026, Invitrogen)