E-MTAB-1178 - Transcription profiling by high throughput sequencing of primordial germ cells from e6.5, e7.5, e8.5 embryos and e7.5 somatic neighbours and Blimp1-KO mice
Released on 1 July 2013, last updated on 17 July 2013
Primordial germ cell mRNA profiles from cells microdissected from e6.5, e7.5 and e8.5 embryos, e7.5 somatic neighbours and Blimp1-KO mice were generated by single cell library construction and sequencing in duplicate using Applied Biosystems SOLiD sequencer. Single cell library construction is described in: Tang f. et. al, Nature Protocols (2010), Vol. 5, p.516.
RNA-seq of coding RNA, cell type comparison, co-expression, development or differentiation, in vivo
A tripartite transcription factor network regulates primordial germ cell specification in mice. Erna Magnúsdóttir, Sabine Dietman, Kazuhiro Murakami, Ufuk Günesdogan, Fuchou Tang, Siqin Bao, Evangelia Diamanti, Kaiqin Lao, Berthold Gottgens and M. Azim Surani. Nat. Cell Biol. (2013), Europe PMC 23851488