9 protocols
AccessionNameType
P-MTAB-26973
labeling
0.5 microgram of digested and purified DNA were labeled by a random primer reaction, using Bioprime DNA Labeling System and either Cy3-dCTP or Cy5-dCTP.
P-MTAB-26977
bioassay_data_transformation
Data preprosessing included filtering of weak and bad spots, lowess normalization, and exclusion of clones showing a SD > 0.2
P-MTAB-26976
feature_extraction
Image analysis was performed with GenePix 6.0 image analysis software
P-MTAB-26969
grow
Tumor biopsies were taken at the time of diagnosis
P-MTAB-26975
image_acquisition
Scanning was performed with an Agilent scanner at 100 pmt
P-MTAB-26971
nucleic_acid_extraction
Genomic DNA was isolated according to a standard protocol, including proteinase K, phenol, chloroform, and isoamylalcohol
P-MTAB-26974
hybridization
The labeled tumor and normal DNA was co-hybridized to the array slides for 42 to 46 hours, using an automated hybridization station.
P-MTAB-26972
nucleic_acid_extraction
Commercially available genomic DNA (Promega, Madison, WI) was used.
P-MTAB-26970
grow
Normal female DNA was used