E-MTAB-1158 - Transcription profiling by array of Entamoeba histolytica trophozoites during growht under different iron concentrations
Released on 30 June 2014, last updated on 3 December 2014
Entamoeba histolytica is the parasite causing amoebiasis, an infectious disease targeting the intestine and liver of humans. The molecules involved in amoeba adaptation to different iron conditions during the invasive process are unknown. To investigate the effects of iron availability on gene expression in E. histolytica, we determined by microarray experiments the gene expression profile of parasites grown in the presence of different iron concentrations. Conditions included low amounts of iron, iron starvation and iron starvation supplemented with hemoglobin (Hb). Genes encoding important proteins involved in iron metabolism, reported in other organism such as bacteria, were identified for the first time in E. histolytica. The influence of iron on the amount of these transcripts was confirmed by quantitative real-time PCR and their protein products were analyzed by Western blots
transcription profiling by array, comparative genome hybridization, dye swap replicated, growth condition design
Gene expression profiling in Entamoeba histolytica identifies key components in iron uptake and metabolism. Nora Adriana Hernandez-Cuevas, Christian Weber, Chung-Chau Hon and Nancy Guillen.