E-MTAB-1148 - Transcription profiling by array of Huh7.5 cells infected with Hepatitis C Virus Jc1

Status
Released on 30 June 2012, last updated on 3 May 2014
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (8)
Description
Huh7/5-2 cells (Binder et al., Hepatology 2007) were mock infected (DMEM) (time points 4 and 48 h) or infected with the chimeric HCV virus Jc1 (Pietschmann et al., PNAS 2006) (all time points). Multiplicity of infection was 15 (TCID50). Cells were lysed after 4, 12, 24, 48 and 72 hours post infection and total cellular RNA was prepared. Mock infected cells serve as controls for the infected samples. The whole experiment was repeated on independent days (2 biological replicates). The dataset was also submitted to GEO (GSE38720).
Experiment types
transcription profiling by array, disease state, time series
Contacts
Citation
Viral immune modulators perturb the human molecular network by common and unique strategies. Andreas Pichlmair, Kumaran Kandasamy, Gualtiero Alvisi,Orla Mulhern, Roberto Sacco, Matthias Habjan, Marco Binder, Adrijana Stefanovic, Carol-Ann Eberle, Adriana Goncalves, Tilmann Burckstummer, Andre C. Muller, Astrid Fauster, Cathleen Holze, Kristina Lindsten, Stephen Goodbourn, Georg Kochs, Friedemann Weber, Ralf Bartenschlager, Andrew G. Bowie, Keiryn L. Bennett, Jacques Colinge & Giulio Superti-Furga. Nature 487(July):486-490 (2012), Europe PMC 22810585
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1148.idf.txt
Sample and data relationshipE-MTAB-1148.sdrf.txt
Raw data (1)E-MTAB-1148.raw.1.zip
Processed data (1)E-MTAB-1148.processed.1.zip
Array designA-AFFY-37.adf.txt
R ExpressionSetE-MTAB-1148.eSet.r
Links