E-MTAB-1147 - Transcription profiling by high throughput sequencing of HNRNPC knockdown and control HeLa cells

Status
Released on 31 January 2013, last updated on 3 May 2014
Organism
Homo sapiens
Samples (6)
Protocols (4)
Description
We performed RNA-seq experiments on two replicate samples from each HNRNPC knockdown (KD1 and KD2) As well as from control HeLa cells. Library preparation was preformed according to mRNA Sequencing Sample Preparation Guide (Illumina, Part # 1004898 REV. D). Reagents were taken from the Illumina sample preparation kit (Illumina, CAT # RS-930-1001). Knockdown and control samples were sequenced together in one flowcell on one and two lanes, respectively.
Experiment types
RNA-seq of coding RNA, cellular modification, co-expression, in vitro
Contact
Citation
Direct Competition between hnRNP C and U2AF65 Protects the Transcriptome from the Exonization of Alu Elements. Kathi Zarnack, Julian Konig, Mojca Tajnik, Inigo Martincorena, Sebastian Eustermann, Isabelle Stevant, Alejandro Reyes, Simon Anders, Nicholas M. Luscombe, Jernej Ule. Cell  (2012), Europe PMC 23374342
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1147.idf.txt
Sample and data relationshipE-MTAB-1147.sdrf.txt
Links