Slides were scanned on an Agilent C Scanner at 3um using the 20bit setting.
Samples were labeled and processed using the Agilent Low Input Quick Amp two colour Cy3 (sample) and Cy5 (mouse reference) RNA labelling kit (Stratagene) according to the manufacturer's instructions.
RNA was extracted from tumours using the miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions.
Tumour-bearing mice were treated with vehicle or 40 mg/kg MLN8054 QD for 3 days.
Mice were enrolled onto this study as tumours were palpable, on a rolling trial basis.
After labelling and purification, samples were loaded onto a SureHyb gasket slide. The Agilent SurePrint G3 Mouse Gene Expression 8x60K array was applied and hybridised in an Agilent hybridisation oven.