6 protocols
AccessionType
image_acquisition
Slides were scanned on an Agilent C Scanner at 3um using the 20bit setting.
hybridization
Samples were labeled and processed using the Agilent Low Input Quick Amp two colour Cy3 (sample) and Cy5 (mouse reference) RNA labelling kit (Stratagene) according to the manufacturer's instructions.
nucleic_acid_extraction
RNA was extracted from tumours using the miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions.
specified_biomaterial_action
Tumour-bearing mice were treated with vehicle or 40 mg/kg MLN8054 QD for 3 days.
grow
Mice were enrolled onto this study as tumours were palpable, on a rolling trial basis.
image_acquisition
After labelling and purification, samples were loaded onto a SureHyb gasket slide. The Agilent SurePrint G3 Mouse Gene Expression 8x60K array was applied and hybridised in an Agilent hybridisation oven.